Academic Publications
My Publications
2022
Perera, PLRA; Perera, WPRT; Liyanage, JA; Premaratne, WAPJ
A comparison of food contamination by heavy metals/metalloids in CKDu prevalent areas and a reference area; A study in Sri Lanka Proceedings Article
In: International Postgraduate Research Conference, pp. 99, Faculty of Graduate Studies, University of Kelaniya Sri Lanka, 2022.
Abstract | Links | BibTeX | Tags: Arsenic, Cadmium, chronic kidney disease, CKDu, Food Safety, Heavy metals, Lead, Nephropathy
@inproceedings{perera2022comparison,
title = {A comparison of food contamination by heavy metals/metalloids in CKDu prevalent areas and a reference area; A study in Sri Lanka},
author = {PLRA Perera and WPRT Perera and JA Liyanage and WAPJ Premaratne},
url = {https://www.researchgate.net/publication/367091378_A_comparison_of_food_contamination_by_heavy_metalsmetalloids_in_CKDu_prevalent_areas_and_a_reference_area_A_study_in_Sri_Lanka},
year = {2022},
date = {2022-12-01},
urldate = {2022-12-01},
booktitle = {International Postgraduate Research Conference},
volume = {2022},
pages = {99},
publisher = {Faculty of Graduate Studies, University of Kelaniya Sri Lanka},
abstract = {During the past two decades, so many studies have been involved in exploring the relationship between the prevalence of chronic kidney disease of unknown etiology (CKDu) and exposure to toxic metals. These studies revealed signs of chronic low levels of toxic metal exposure in Sri Lanka. To assess the risk of negative health effects and propose appropriate public health interventions, novel research studies will be very beneficial. This assessment was performed to study the toxic metal contamination status in domestic rice, leafy vegetables, legumes, and citrus fruits in the CKDu prevalence area in north central province in Sri Lanka and to compare those levels with a reference area in the Eastern province in Sri Lanka where CKDu is not prevalent. CKDu hot spots areas Maradankulama-Mahakanadarawa in the Anuradhapura district, Sri Lanka was selected for sampling and Buddhangala Grama Niladhari area in Ampara district was selected as a reference site. Food samples, including (Oryza sativa; n=25, Centella asiatica; n=25, Citrus crenatiflora; n=18, Vigna radiata; n=10) were collected from the stratified sampling method in both areas and the collected food samples were digested by microwave digestion according to the standard procedures and the concentrations of metal ions in food samples, including Cadmium, Nickel, Chromium, Copper, Iron, Manganese, Lead, Zinc, Arsenic, and Calcium, were measured using Inductive Coupled Plasma technique (ICP). As a results of the study, mean As and Pb concentrations of Oryza sativa (As-0.15 mg/kg, Pb-3.10 mg/kg) and Citrus crenatiflora (As-0.15 mg/kg, Pb-0.47 mg/kg), Pb (0.65 mg/kg) and Cd (0.05 mg/kg) content in Centella asiatica, and mean concentrations of As (0.14 mg/kg), Pb (9.25 mg/kg) and Cu (57.8 mg/kg) in Vigna radiata have exceeded the permissible limits given by the FAO and WHO, 2011: As-0.1 mg/kg, Pb-0.1 mg/kg, Cd-0.05 mg/kg, Cr-2.3 mg/kg, Cu-40 mg/kg. However, none of the food samples collected from the reference areas has exceeded the permissible limits of analyzed heavy metals/metalloids. THQ (Target Hazard Quotient) values of Cr have exceeded the threshold values for all food species. Furthermore, Pb is also regarded as a toxic element and THQ values of Pb in Oryza sativa and Vigna radiate are noticeably high. Meanwhile, THQ values of the food samples in the reference areas have not exceeded the threshold values. According to the outcome of the study, there is a noticeable difference reported in toxic metals contamination status between the CKDu-prone areas and the reference area. Therefore, the consumption of analyzed rice, leafy vegetables, and legumes was estimated to be risky, and their regular consumption may boost the likelihood of CKDu prevalence in those areas. Further studies are required to monitor and assess heavy metals and metalloids in soil, and fertilizers to find the pathways of contamination of food by toxic metals.},
keywords = {Arsenic, Cadmium, chronic kidney disease, CKDu, Food Safety, Heavy metals, Lead, Nephropathy},
pubstate = {published},
tppubtype = {inproceedings}
}
Perera, PLRA; Perera, WPRT; Liyanage, JA; Premaratne, WAPJ
Health risk analysis of toxic metals via consumption of rice and other food species grown in Mahakanadarawa-Maradankulama areas in Anuradhapura district; A study in Sri Lanka Proceedings Article
In: International Conference on Applied and Pure Sciences (ICAPS), pp. 76, Faculty of Science, University of Kelaniya Sri Lanka, 2022.
Abstract | Links | BibTeX | Tags: CKDu, Food Safety, Health Risk, Heavy metals, Nephropathy
@inproceedings{perera2022health,
title = {Health risk analysis of toxic metals via consumption of rice and other food species grown in Mahakanadarawa-Maradankulama areas in Anuradhapura district; A study in Sri Lanka},
author = {PLRA Perera and WPRT Perera and JA Liyanage and WAPJ Premaratne},
url = {https://www.researchgate.net/publication/364622134_Health_risk_analysis_of_toxic_metals_via_consumption_of_rice_and_other_food_species_grown_in_Mahakanadarawa_-Maradankulama_areas_in_Anuradhapura_district_A_study_in_Sri_Lanka},
year = {2022},
date = {2022-10-01},
urldate = {2022-10-01},
booktitle = {International Conference on Applied and Pure Sciences (ICAPS)},
pages = {76},
publisher = {Faculty of Science, University of Kelaniya Sri Lanka},
abstract = {Chronic Kidney Disease of unknown etiology (CKDu) is a heterogeneous disorder that impairs kidney functions and structure. Even though a number of studies were conducted on this issue, a specific reason has not yet been discovered. Unexpected contaminants have harmed crop quality in recent decades, threatening food security and human health. This study was designed to assess the status of toxic metal contamination of frequently consuming food species in selected Maradankulama-Mahakanadarawa Grama Niladari (GN) divisions to evaluate the link between CKDu prevalence and food safety in the selected areas. Maradankulama-Mahakanadarawa GN divisions in Anuradhapura district, Sri Lanka, were selected as the sampling areas. The sampling procedure was conducted in April (2022). Food samples, including (rice grains of Oryza sativa; n=25, leaves of Centella asiatica; n=25, fruits of Citrus crenatiflora; n=18, grains of Vigna radiate; n=10) were according to the random stratified sampling method and the collected food samples were digested by microwave digestion. (added 8.0 mL of conc. Nitric acid to 0.2 g of food sample and digested) The concentrations of metal ions in food samples, including Cadmium, Nickel, Chromium, Copper, Iron, Manganese, Lead, Zinc, Arsenic, and Calcium, were measured using the Inductive Coupled Plasma technique (ICP). Statistical analysis was done using SPSS Statistics Software. Accordingly, mean As and Pb concentrations of Oryza sativa (As-0.15 mg/kg, Pb-3.10 mg/kg) and Citrus crenatiflora (As-0.15 mg/kg, Pb-0.47 mg/kg), Pb (0.65 mg/kg) and Cd (0.05 mg/kg content in Centella asiatica, and mean concentrations of As (0.14 mg/kg), Pb (9.25 mg/kg) and Cu (57.8 mg/kg) in Vigna radiate exceeded the permissible limits given by the FAO and WHO, 2011 (As-0.1 mg/kg, Pb-0.1 mg/kg, Cd-0.05 mg/kg, Cr-2.3 mg/kg, Cu-40 mg/kg). In addition to Pb, Cd, As, Cr and Cu, other detected metals have not exceeded the permissible limits in all samples. According to the Target Hazard Quotient (THQ) values of Cr exceeded the threshold value of 1 for all food species. In addition to that, Pb is also considered a toxic element, and THQ values of Pb in Oryza sativa and Vigna radiate reported noticeably high values. The total EDIs (Estimated Daily Intake) of Cr, Ni, Cd, As, Cu and Pb for all selected food samples studied have not exceeded the maximum tolerable daily intake consumption of analyzed rice, leafy vegetables, and legumes was deemed unsafe, and their regular consumption may cause a risk for the prevalence of CKDu in selected areas.},
keywords = {CKDu, Food Safety, Health Risk, Heavy metals, Nephropathy},
pubstate = {published},
tppubtype = {inproceedings}
}